Lentinula edodes, one of the most popular, edible mushroom species with a high content of proteins and polysaccharides as well as unique aroma, is widely cultivated in many asian countries, especially in china, japan and korea. Cellobiose dehydrogenase cdh gene transcripts were quantified by reverse transcriptionpcr rtpcr in cultures of phanerochaete chrysosporium supplemented with various cello and xylooligosaccharides in order to elucidate the mechanism of enhanced cdh production in xylancellulose culture. The whiterot basidiomycete fungus phanerochaete chrysosporium agaricomycetes is a model species that produces potent wooddegrading enzymes. The wood decay basidiomycete phanerochaete chrysosporium was grown under standard ligninolytic or cellulolytic conditions and subjected to whole genome expression microarray analysis and liquid chromatographytandem mass spectrometry of extracellular proteins. Naidu department of microbiology and public health, and nsf center for microbial ecology, michigan state university. The genome of phanerochaete chrysosporium was sequenced and shows the genetic potential to make over 100 cytochrome p450 monooxygenases. Genome sequence of the lignocellulose degrading fungus phanerochaete chrysosporium strain rp78 vol 22, pg 695, 2004. White rot fungi have been used in bioremediation efforts to break down potentially harmful chemicals in soil and in water. The completed genome sequences and genomics databases currently available for plants, animals and fungi provide a scaffold for addressing the biological role of the impressive gene collection in these organisms. The proteinencoding sequence is interrupted by eight introns which conform to the universal gtag splicing rule observed for the. Phanerochaete chrysosporium atcc 20696 has a catabolic ability to degrade lignin. Phanerochaete chrysoporiums genome consists of approximately 29. We have sequenced the 30million basepair genome of. Biochemical characterization of cyp505d6, a selfsufficient.
Taxonomy browser phanerochaete chrysosporium ncbi nih. A high quality draft genome sequence has been generated for the lignocellulosedegrading basidiomycete phanerochaete chrysosporium martinez et al. Target of rapamycin pathway in the whiterot fungus. Metadata analysis of phanerochaete chrysosporium gene. Genomic analysis provides structural, comparative, and functional information. This is the first basidiomycete genome to be sequenced. They are sent to a computer by website operators or third parties. Glg2 ligninase lg2 precursor phanerochaete chrysosporium. Phanerochaete chrysosporium, a crust fungus important in. A cluster of multicopper oxidase genes mco1, mco2, mco3, mco4 from the lignindegrading basidiomycete phanerochaete chrysosporium is described. Cookies are small text files that contain a string of characters and uniquely identifies a browser. Jgi decodes wood and toxic wastedegrading fungus genome. Phanerochaete chrysosporiumwhite rot fungushas been reported as an effective for bioremediation of different hazardous compounds.
To elucidate the genetic basis of softwood bioconversion by a whiterot fungus, the present study reports the p. Gene annotation provided crucial information about the location and function of proteinencoding genes. Characterization of a multicopper oxidase gene cluster in. As of very recently, phanerochaete chrysosporium is the first wooddecay basidiomycete to have its genome sequenced. Cellotriose and cellotetraose induced cdh expression, while xylobiose and xylotriose induced. A single matingtype locus composed of homeodomain genes. Research article open access comparative genomics of the. A ligninase gene has been cloned from a phanerochaete chrysosporium genomic dna library. Comparative genomics of ceriporiopsis subvermispora and. Efficient lignin depolymerization is unique to the wood decay basidiomycetes, collectively referred to as white rot fungi. Martinez d1, larrondo lf, putnam n, gelpke md, huang k, chapman j, helfenbein kg, ramaiya p, detter jc, larimer f, coutinho pm, henrissat b, berka r, cullen d, rokhsar d. May 02, 2004 jgi decodes wood and toxic wastedegrading fungus genome walnut creek, cathe united states department of energy doe joint genome institute jgi announces today the publication of a highquality draft genome sequence of the white rot fungus, phanerochaete chrysosporium. By exploiting the draft genome sequence, we reevaluated the mating system of p. Transcriptome and secretome analyses of phanerochaete.
Nucleotide sequence of a new lignin peroxidase gene glg3 from the whiterot fungus, phanerochaete chrysosporium p. Bioremediation of organic pollutants using phanerochaete. Gene expression profiling and enzymatic function of. Genome sequence of the lignocellulose degrading fungus. On selected data sets, the current status of which will be indicated in the table, the doe joint genome institute reserves the exclusive right to publish, not more than 12 months following completion of the draft sequencing phase, the assembly and sequence along with an initial genomescale analysis. The whiterot fungus phanerochaete chrysosporium encodes 149 cytochrome p450 cyp genes in its genome. Genome sequence of the lignocellulose degrading fungus phanerochaete chrysosporium strain rp78 skip to main content thank you for visiting. We determined the 39mb draft genome sequence of this fungus, comprising,560 predicted gene models.
Citeseerx document details isaac councill, lee giles, pradeep teregowda. Computational analysis of the phanerochaete chrysosporium v2. Therefore, phanerochaete chrysosporium is the first member of the basidiomycetes to have its complete genome sequenced. To elucidate the genetic basis of this technologically important behavior, we have sequenced the thirty million basepair genome of the white rot fungus phanerochaete chrysosporium to high draft using a whole genome shotgun method. Jun 14, 2005 phanerochaete chrysosporium, the model white rot basidiomycetous fungus, has the extraordinary ability to mineralize to co2 lignin and detoxify a variety of chemical pollutants. Phanerochaete chrysosporium genomics sciencedirect. This genome sequence will certainly help with manipulations of phanerochaete in future biotechnological applications. Biochemical characterization and transcriptional analysis of.
Its cytochrome p450 monooxygenases have recently been implied in several of these biotransformations. Research article open access cloning, expression and. The mating system of the species has been difficult to characterize due to its cryptic fruiting habit and lack of clamp connections in the heterokaryotic phase. In this chapter, the life cycle of white rot fungus and the conditions required for its growth so as to make effective bioremediation of compounds have been cited. Gene expression profiling and enzymatic function of phanerochaete chrysosporium cytochrome p450s involved in the metabolism of benzoapyrene. To elucidate the genetic basis of this technologically important behavior, we have sequenced the thirty million basepair genome of the white rot fungus. Pdf genome sequence of the lignocellulose degrading fungus. Nucleotide sequencing of the gene has revealed that the ligninase structural gene contains 1116 bp of the proteinencoding sequence, of which 84 bp encode the signal peptide. Comparative genomics of the whiterot fungi, phanerochaete carnosa and p. You can read more about the genome sequencing in the june 2004 issue of nature biotechnology.
To reveal the genetic repertoire of white rot fungi we have sequenced the genome of p. The four genes share the same transcriptional orientation within a 25 kb region. Phanerochaete chrysosporium, were mainly isolated from hardwoods. Cloning of a cdna from phanerochaete chrysosporium encoding an arylalcohol dehydrogenase using the amino acid sequence coded by a previously cloned aad orf from phanerochaete chrysosporium pc strain ogc101 20 as query, a blast alignment was performed against the translated predicted orfs of the genome sequence of p. The functions of many of these genes or their target substrates are still. We have sequenced the 30million basepair genome of phanerochaete chrysosporium strain rp78 using a whole genome shotgun approach. Genome sequence of the lignocellulose degrading fungus phanerochaete chrysosporium strain rp78.
Here, we report wholegenome sequencing used to identify genes related to lignin modification. As a white rot fungus with lignocellulose degradation ability, l. Genome sequence of the edible cultivated mushroom lentinula. Nucleotide sequence of a new lignin peroxidase gene glg3 from. Lipa ligninase a precursor phanerochaete chrysosporium. Phanerochaete chrysosporium is a whiterot fungus whose ligninolytic and xenobiotic metabolism has been extensively studied 1 9. Phanerochaete chrysosporium simultaneously degrades lignin and cellulose, whereas the closely related species, ceriporiopsis subvermispora, also depolymerizes lignin but may do so with relatively little cellulose degradation. Computational analysis of the phanerochaete chrysosporium. Transcriptional response of the cellobiose dehydrogenase gene. Phanerochaete chrysosporium whiterot fungus sporotrichum pruinosum. Comparative genomics of the whiterot fungi, phanerochaete. Chigu nomathemba loice 1, hirosue sinji 2, nakamura chie 2, teramoto hiroshi 2, ichinose hirofumi 2 and wariishi hiroyuki 1, 3. Analysis of the genome in the context of previously established genetics and physiology is presented.
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